![]() ![]() 9įrom an analytical point of view, the determination of cell chirality based on alignment bias is more accessible on micropatterned lines, compared to rings. Likewise, it has also been observed that there is no cell chirality bias exhibited on circle or square patterned geometries. ![]() Previous studies have reported that equivalent chirality bias was observed on both geometries, however no quantitative data was provided. 9,13–18 Typically, pattern widths between 100 and 300 μm have previously been used. 9,12 However, comparison between different studies is often complicated by the use of different micropattern geometries ( e.g., lines or rings) and different pattern widths. 9,11 Furthermore, the method can be used to produce a large number of microscale patterns to enable high-throughput studies with high statistical power.Ĭell chirality offers an attractive comparative property of cells across cell studies due to its binary-switch nature. 7–10 Micropatterned substrates are a suitable technology for such studies, as they provide a simple mean to achieve well-defined edges that are the required cue for the cells to display their chirality bias. Micropatterned substrates have been used to study cell chirality in vitro, where cells have been cultured on well-defined boundaries in the micrometre scale to elucidate the mechanisms of cell chirality development. Human brain microvascular endothelial cells Human umbilical vein endothelial cells (HUVEC) 9 This study has since been extended and this chirality bias has now been reported on various cell types ( Table 1). determined that cell chirality is cell type specific, when they were the first to observe that different cell types or disease states express different left-right orientation biases. 5 However, in vitro both vertebrates and human cell chirality has been observed, demonstrating an inherent cell chirality under specific culture conditions. 2Ĭell chirality has been observed in vivo, but only in invertebrates. 3,4 It has been proposed that cells possess an inherent chirality which translates into the formation of asymmetric tissue and organs. 1,2 Proper establishment of left-right asymmetry is required for normal embryonic development and function maintenance, and defects in asymmetry often result in birth defects. However, on the inside, it is far from symmetric, where the organs’ shape and position present left-right asymmetry, i.e., so-called chirality. Introduction Externally, humans exhibit a bilateral symmetric body. This work serves as a guide to determine optimal micropattern width for further investigations on cell chirality bias and its prominence in e.g., disease states or upon exposure to toxic substances. We also observed that this exhibited chirality bias varied across the line width. Overall, this cell type exhibited a negative chirality bias on micropatterned lines ranging from 10 μm to 400 μm in width, where the negative bias was most pronounced on the 100 μm wide lines. Here, we utilized micropatterned RGD-peptide lines on hyaluronic acid hydrogels to investigate the effect of the micropattern width on the exhibited cell chirality bias of brain microvascular endothelial cells. However, micropattern geometry and size often varies across different studies, making it challenging to compare results. Cell chirality is often studied using micropatterned in vitro models. Left-right asymmetry is a conserved property in nature and observed in the human body, a property known as cell chirality. ![]()
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